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KMID : 0903619840250020164
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Journal of the Korean Society for Horticultural Science
1984 Volume.25 No. 2 p.164 ~ p.169
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Physiological Characteristics of cymbidium Protocorm Cultured in Vitro
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Abstract
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In this communication main emphasis is given upon the origin of organogenesis from cultured protocorm which showed different morphogenetic capacity after subcultured more 30 times in order to obtain physiological and morphological uniform protocorms for experiment, and the differences of isoperoxidase pattern among differentiated tissues. Initial protocorms were obtained from shoot tip culture of Cymbidium elcapitan `Conquestador´ and Cym. balkis `St. Anton´
Adventitious shoots and roots were originated in the cortex and pericycle of cultured protocorms respectively, whereas development of new protocorm-like body was originated from the one or two layers of epidermis cells in protocorm.
Cells in the non-shoot-forming protocorms contained several starch grains, and leaf primordia occurred abnormally on one side of the axis.
In isoperoxidase patterns of protocorm, low level of activity of the most anodic bands was observed in the shoot-forming protocorm while fast-moving anodic isoperoxidase was observed in the non-shoot-forming protocorm. Several cathodic isoperoxidases(p1-p5) were observed in the shoot tissue compared with root tissue.
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